• LEHU乐虎

    tRNA碱基修饰检测

    • 简介
    • 分析种类
    • 结果展示

    • Aksomics（原LEHU乐虎生物）的质谱检测平台能够为客户给予全面而准确的tRNA修饰谱。利用Agilent质谱仪，检测36种修饰碱基在各个样品中的修饰丰度。


      服务特色：

      ● 优化的前期处理与Agilent优质设备 

      ● 全面而准确的碱基修饰分析


      分析流程：

      LC-MS tRNA修饰检测服务流程图

      图释：LC-MS tRNA修饰检测服务流程图。


    • Number Nucleoside Symbol Number Nucleoside Symbol
      01 3′-O-methyladenosine 3′-OMeA 27 3'-O-methyluridine 3'-OMeU
      02 2′-O-methylcytidine Cm 28 5-methyl-2-thiouridine m5s2U
      03 3-methylcytidine m3C 29 5-methoxyuridine mo5U
      04 5-methylcytidine m5C 30 pseudouridine Ψ
      05 N6-isopentenyladenosine i6A 31 2'-O-methylinosine Im
      06 5,2'-O-dimethylcytidine m5Cm 32 3-methyluridine m3U
      07 1-methyladenosine m1A 33 1-methylpseudouridine m1Ψ
      08 2-thiocytidine s2C 34 5-hydroxymethylcytidine hm5C
      09 N2,N2,7-trimethylguanosine m2,2,7G 35 5,2'-O-dimethyluridine m5Um
      10 N4-acetyl-2'-O-methylcytidine ac4Cm 36 N6-threonylcarbamoyladenosine t6a
      11 N6-methyladenosine m6A 37 2-methylthio-N6-threonylcarbamoyladenosine ms2t6A
      12 3'-O-methylcytidine 3′-OMeC 38 5-carboxymethyluridine cm5U
      13 2'-O-methyladenosine Am 39 5-methoxycarbonylmethyl-2-thiouridine mcm5s2U
      14 N2, N2-dimethylguanosine m22G 40 5-Methoxycarbonylmethyluridine mcm5U
      15 5'-O-methylthymidine 5′-OMeT 41 2-methylthio-N6-isopentenyladenosine ms2i6A
      16 2′-O-methyluridine Um 42 Peroxywybutosine o2Yw
      17 inosine I 43 5-taurinomethyl-2-thiouridine tm5s2U
      18 2′-O-methylguanosine Gm 44 5-oxyacetic acid uridine cmo5U
      19 1-methylguanosine m1G 45 5-carbamoylmethyuridine ncm5U
      20 7-methylguanosine m7G 46 Queuosine Q
      21 N2-methylguanosin m2G 47 5-taurinomethyluridine tm5U
      22 3'-O-methylinosine 3'-OMeI 48 5-formyl-2′-O-methylcytidine f5Cm
      23 2-thiouridine s2U 49 dihydrouridine D
      24 4-thiouridine s4U 50 5-formylcytidine f5c
      25 5-methyluridine m5U 51 wybutosine yW
      26 N4-acetylcytidine ac4C 52 5-methoxycarbonylmethyl-2'-o-methyluridine mcm5Um

    • 1. 修饰定量分析

      tRNA修饰数量分析图

      图释：tRNA修饰数量分析图。


      2. 总离子流程图

      tRNA总离子流程图

      图释：总离子流程图